https://eln.excellgen.com/index.php?action=history&feed=atom&title=1813_1811 1813 1811 - Revision history 2026-10-18T15:58:28Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1813_1811&diff=10343&oldid=prev Paul.xu: Created page with "September, 2013 Image:FLP_vPol_purification_9_2013.jpg<br> {| width="472" height="244" cellspacing="1" cellpadding="1" border="1" |- | <br> | Desc<br> | CloneID<br> | Name<..." 2013-09-28T18:02:54Z <p>Created page with &quot;September, 2013 <a href="/index.php?title=File:FLP_vPol_purification_9_2013.jpg" title="File:FLP vPol purification 9 2013.jpg">Image:FLP_vPol_purification_9_2013.jpg</a>&lt;br&gt; {| width=&quot;472&quot; height=&quot;244&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; |- | &lt;br&gt; | Desc&lt;br&gt; | CloneID&lt;br&gt; | Name&lt;...&quot;</p> <p><b>New page</b></p><div>September, 2013<br /> <br /> [[Image:FLP_vPol_purification_9_2013.jpg]]&lt;br&gt;<br /> <br /> {| width=&quot;472&quot; height=&quot;244&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> | Desc&lt;br&gt;<br /> | CloneID&lt;br&gt;<br /> | Name&lt;br&gt;<br /> |-<br /> | 1&lt;br&gt;<br /> | Fermentas PageRuler Plus&lt;br&gt;<br /> | &lt;br&gt;<br /> | &lt;br&gt;<br /> |-<br /> | 2&lt;br&gt;<br /> | &quot;purified&quot; Flp dialysed&lt;br&gt;<br /> | 1813&lt;br&gt;<br /> | FLP&lt;br&gt;<br /> |-<br /> | 3&lt;br&gt;<br /> | FLP Ni HisTrap HP eluate&lt;br&gt;<br /> | 1813&lt;br&gt;<br /> | FLP&lt;br&gt;<br /> |-<br /> | 4&lt;br&gt;<br /> | <br /> FLP Haparin HyperD Fraction 57 (1M NaCl elute)<br /> <br /> Nothing is eluted from 0.1 to 1 M NaCl&lt;br&gt;<br /> <br /> | 1813&lt;br&gt;<br /> | FLP&lt;br&gt;<br /> |-<br /> | 5&lt;br&gt;<br /> | &amp;nbsp;50 mM arginine wash, 5 ml Ni HisTrap HP column&lt;br&gt;<br /> | 1811&lt;br&gt;<br /> | Vpol&lt;br&gt;<br /> |-<br /> | 6&lt;br&gt;<br /> | 100 mM arginine wash, 5 ml Ni HisTrap HP column&lt;br&gt;<br /> | 1811&lt;br&gt;<br /> | Vpol&lt;br&gt;<br /> |-<br /> | 7&lt;br&gt;<br /> | 150 mM arginine wash, 5 ml Ni HisTrap HP column&lt;br&gt;<br /> | 1811&lt;br&gt;<br /> | Vpol&lt;br&gt;<br /> |-<br /> | 8&lt;br&gt;<br /> | 20 to 300 mM Imidazole gradient elute, fraction #6, 5 ml Ni HisTrap HP column&lt;br&gt;<br /> | 1811&lt;br&gt;<br /> | Vpol&lt;br&gt;<br /> |-<br /> | 9&lt;br&gt;<br /> | His-MBP-Taq-Muts contaminated by some FLP&lt;br&gt;<br /> | 211&lt;br&gt;<br /> | His-MBP-Taq-Muts &lt;br&gt;<br /> |-<br /> | 10&lt;br&gt;<br /> | His-MBP-Taq-Muts eluted from amylose beads&lt;br&gt;<br /> | 211&lt;br&gt;<br /> | His-MBP-Taq-Muts &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt;<br /> <br /> [[Category:Protein_Purification]]<br /> <br /> &lt;br&gt;</div> Paul.xu