https://eln.excellgen.com/index.php?action=history&feed=atom&title=1816_pCold-MBP-%28E3C%29-Enterokinase 2026-10-19T00:25:03Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1816_pCold-MBP-(E3C)-Enterokinase&diff=10003&oldid=prev 2013-08-12T20:41:40Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1816 pCold-MBP-(E3C)-Enterokinase&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of1816 pCold-MBP-(E3C)-Enterokinase <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;08, 06, 2013&lt;br&gt; <br /> <br /> &#039;&#039;&#039;Notebook:&amp;nbsp;&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID: 1816&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters: &#039;&#039;&#039;Shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === Cloning ===<br /> <br /> subclone from 1814 pMAL-p5E-Enterokinase or 1815 pMAL-c5E-Enterokinase to 1804 pCold-MBP-(E3C) using Bsa1 + BamH1 &lt;br&gt; <br /> <br /> cut 1814 the middle band=&amp;gt;2000bp <br /> <br /> should be 1884 bp&lt;br&gt; <br /> <br /> [[Image:Digest for1816.JPG]]&lt;br&gt; <br /> <br /> === &amp;nbsp;Colony screen ===<br /> <br /> 8/10/2013: make miniprep, digest with Kpn1 to screen, clone #5 could be the desired clone&lt;br&gt; <br /> <br /> === ===<br /> <br /> [[Image:1816 digestion kpn1.JPG]]&lt;br&gt; <br /> <br /> === ===<br /> <br /> 8/112013: digest clone #5 with enzymes to verify&lt;br&gt; <br /> <br /> [[Image:1816 digestion 8-11 13.JPG]] <br /> <br /> === ===<br /> <br /> lane 2: should be Nco1 + Xho1, not Nde1 + Xho1&lt;br&gt; <br /> <br /> [[Image:1816-5DIGEST WITHNdeI+BamHI.JPG]]&lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div>

Paul.xu