https://eln.excellgen.com/index.php?action=history&feed=atom&title=1818-pT7A30-TthDNALigase 1818-pT7A30-TthDNALigase - Revision history 2026-10-18T15:35:12Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1818-pT7A30-TthDNALigase&diff=10062&oldid=prev Paul.xu at 19:51, 16 August 2013 2013-08-16T19:51:25Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1818-pT7A30-TthDNALigase&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1818-pT7A30-TthDNALigase <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-8-13-----2013-8-16 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1818&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1818 clone 8-6-2013.JPG]] <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; width=&quot;515&quot;<br /> |-<br /> | lane&lt;br&gt; <br /> | 1&lt;br&gt; <br /> | 2&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | 1035, pET28b-Tth-Ligase-K294R&lt;br&gt; <br /> | 1727 pT7A30&lt;br&gt;<br /> |-<br /> | enzymes&lt;br&gt; <br /> | colspan=&quot;2&quot; | &amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp; Nde1 + EcoR1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-8-16<br /> <br /> Make miniprep and digest with Nde1 +BamH1/ BamH1+EcoR1<br /> <br /> <br /> <br /> [[Image:1818digest_8-16-2013.JPG]]<br /> <br /> <br /> <br /> clone 2 digest with Nco1+Hind111<br /> <br /> [[Image:1818-2_digest_8-16-2013.JPG]]<br /> <br /> clone 2 for stock<br /> <br /> &lt;br&gt; <br /> <br /> &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; <br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div> Paul.xu