https://eln.excellgen.com/index.php?action=history&feed=atom&title=1819-pT7A30-TthDNALigase-C-6XHis 1819-pT7A30-TthDNALigase-C-6XHis - Revision history 2026-10-18T14:43:51Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1819-pT7A30-TthDNALigase-C-6XHis&diff=10099&oldid=prev Paul.xu at 14:54, 21 August 2013 2013-08-21T14:54:12Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1819-pT7A30-TthDNALigase-C-6XHis&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1819-pT7A30-TthDNALigase-C-6XHis <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-8-13--- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1819&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1812+1819PCR 8-9-2013.JPG]]&lt;br&gt; <br /> <br /> PCR the c-terminus fragment &lt;br&gt; <br /> <br /> template: 1035pET28b-Tth-Ligase-K294R&lt;br&gt; <br /> <br /> Tth-Ligase-Nco1-F &lt;br&gt; <br /> <br /> Tth-Ligase-HindIII-R&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> [[Image:1819+1825clone 8-19-2013.JPG]]&lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; width=&quot;385&quot;<br /> |-<br /> | lane&lt;br&gt; <br /> | 1&lt;br&gt; <br /> | 2&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | PCR product&lt;br&gt; <br /> | 1818 pT7A30-TthDNALigase&lt;br&gt;<br /> |-<br /> | enzymes&lt;br&gt; <br /> | colspan=&quot;2&quot; | Nco1 + HindIII&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-8-21<br /> <br /> Make miniprep and digest with Nco1 + HindIII/Xho1&lt;br&gt; <br /> <br /> [[Image:1819digest_8-21-2013.JPG]]<br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div> Paul.xu