https://eln.excellgen.com/index.php?action=history&feed=atom&title=1821_pT7A30-TthPol-C6XHis 2026-10-18T19:42:10Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1821_pT7A30-TthPol-C6XHis&diff=10042&oldid=prev 2013-08-15T14:54:35Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1821 pT7A30-TthPol-C6XHis&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1821 pT7A30-TthPol-C6XHis <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;08, 14, 2013&lt;br&gt; <br /> <br /> &#039;&#039;&#039;Notebook:&amp;nbsp;&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID: 1821&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters: &#039;&#039;&#039;Shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === Cloning ===<br /> <br /> PCR from ATCC Genomic DNA from Thermus thermophilus Strain DSM 7039 [HB27] ATCC BAA-163D-5 <br /> <br /> [[Image:1821PCR.JPG]]&lt;br&gt; <br /> <br /> digest with Nde1 + Not1&lt;br&gt; <br /> <br /> [[Image:Digest for 1821.JPG]]&lt;br&gt; <br /> <br /> === &amp;nbsp;Colony screen ===<br /> <br /> &amp;nbsp;make miniprep&lt;br&gt;digestion with NdeI+Nco1 will get a small fragment+ a large fragment&lt;br&gt; <br /> <br /> [[Image:1821digest2.JPG]][[Image:1821digest1.JPG]]<br /> <br /> &lt;br&gt; <br /> <br /> === ===<br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div>

Paul.xu