https://eln.excellgen.com/index.php?action=history&feed=atom&title=1823_pCold-HTNCre 1823 pCold-HTNCre - Revision history 2026-10-18T16:28:49Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1823_pCold-HTNCre&diff=10031&oldid=prev Paul.xu at 22:10, 13 August 2013 2013-08-13T22:10:44Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1823 pCold-HTNCre&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1823 pCold-HTNCre <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;08, 12, 2013&lt;br&gt; <br /> <br /> &#039;&#039;&#039;Notebook:&amp;nbsp;&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID: 1823&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters: &#039;&#039;&#039;Shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === Cloning ===<br /> <br /> subclone from 1708 pT7A3-HTNCre TO 1804 pCold-MBP-(E3C)using Nco1 + Xho1 <br /> <br /> [[Image:DIGEST FOR1823.JPG]]&lt;br&gt; <br /> <br /> === &amp;nbsp;Colony screen ===<br /> <br /> 8/13/2013: make miniprep, digest with NheI+BamHI&lt;br&gt;<br /> <br /> the parental vector 1708 does not have NheI site, 1804 should get a 1344bp band, 1823 should get a 614bp band<br /> <br /> [[Image:1823digest2.JPG]]&lt;br&gt;&lt;br&gt;<br /> <br /> &lt;br&gt; <br /> <br /> === ===<br /> <br /> <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Paul.xu