https://eln.excellgen.com/index.php?action=history&feed=atom&title=1827_1828_1829_1830_1831 1827 1828 1829 1830 1831 - Revision history 2026-10-18T14:38:40Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1827_1828_1829_1830_1831&diff=10097&oldid=prev Paul.xu at 13:37, 21 August 2013 2013-08-21T13:37:41Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1827 1828 1829 1830 1831&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1827 1828 1829 1830 1831 <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;08, 20, 2013&lt;br&gt; <br /> <br /> &#039;&#039;&#039;Notebook:&amp;nbsp;&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID: 1827 1828 1829 1830 1831&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters: &#039;&#039;&#039;Shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === Cloning ===<br /> <br /> 1827 pT7A30-FN3K &amp;nbsp; &amp;nbsp; subclone from 1822 pT7A3-MBP-(E3C)-FN3K TO 1727pT7A30 <br /> <br /> 1828 pT7A3-8H-FN3K&amp;nbsp; subclone from 1822 pT7A3-MBP-(E3C)-FN3K TO 1725pT7A3-8H <br /> <br /> 1829 pCold-FN3K&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp; subclone from 1757 pT7A3-MBP-(3C)-FN3K TO v19 pET28-cold <br /> <br /> 1830 pET28b-FN3K&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp; subclone from 1757 pT7A3-MBP-(3C)-FN3K TO V12 pET28b <br /> <br /> 1831 pET30-FN3K&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp; subclone from 1809 pCold-MBP-(E3C)-FN3K TO V15 pET30b <br /> <br /> [[Image:Digest for 1827 1828 1829 1830 1831.JPG]][[Image:1757 1809 1822digest.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> === &amp;nbsp;Colony screen ===<br /> <br /> digest with Nde1 + Xho1&amp;nbsp; <br /> <br /> [[Image:1827_1828_1829_1830digest.JPG‎ ]][[Image:1830_1831digest.JPG‎ ]]&lt;br&gt; <br /> <br /> &lt;br&gt; &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Paul.xu