https://eln.excellgen.com/index.php?action=history&feed=atom&title=1827_1828_1829_1830_1831_1823 1827 1828 1829 1830 1831 1823 - Revision history 2026-10-18T02:21:33Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1827_1828_1829_1830_1831_1823&diff=10115&oldid=prev Paul.xu at 20:38, 23 August 2013 2013-08-23T20:38:51Z <p></p> <p><b>New page</b></p><div>== Protein Expression ==<br /> <br /> 2013/8/22&lt;br&gt; SHANYI <br /> <br /> [[Image:1827 1828 1829 1830 1831 1823.jpg]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; width=&quot;982&quot;<br /> |-<br /> | Lane&lt;br&gt; <br /> | Vector Name&lt;br&gt; <br /> | Name&lt;br&gt; <br /> | Clone ID&lt;br&gt; <br /> | Host&lt;br&gt; <br /> | Induction&lt;br&gt; <br /> | Purification&lt;br&gt; <br /> | pI/Mw&lt;br&gt; <br /> | Expression &lt;br&gt;<br /> |-<br /> | 1&lt;br&gt; <br /> | rowspan=&quot;2&quot; | pT7A30-FN3K&lt;br&gt; <br /> | rowspan=&quot;9&quot; | FN3K&lt;br&gt; <br /> | rowspan=&quot;2&quot; | 1827&lt;br&gt; <br /> | rowspan=&quot;2&quot; | &amp;nbsp;&amp;nbsp; 5 ml, <br /> BL21DE3pRare2pLysS &lt;br&gt; &lt;br&gt; <br /> <br /> | 0.2 mM IPTG &amp;nbsp;&amp;nbsp; 37oC,4h&lt;br&gt; <br /> | rowspan=&quot;2&quot; | Ni beads &lt;br&gt; <br /> | rowspan=&quot;2&quot; | 6.95 / 36349.49 &lt;br&gt; <br /> | &lt;br&gt; &lt;br&gt;<br /> |-<br /> | 2&lt;br&gt; <br /> | <br /> 0.2 mM IPTG&amp;nbsp;&amp;nbsp; RT o/n&lt;br&gt; <br /> <br /> | low&lt;br&gt;<br /> |-<br /> | 3&lt;br&gt; <br /> | rowspan=&quot;2&quot; | pT7A3-8H-FN3K&lt;br&gt; <br /> | rowspan=&quot;2&quot; | 1828&lt;br&gt; <br /> | rowspan=&quot;2&quot; | &amp;nbsp;&amp;nbsp; 5 ml, <br /> BL21DE3pRare2pLysS &lt;br&gt; &lt;br&gt; <br /> <br /> | 0.2 mM IPTG&amp;nbsp;&amp;nbsp;&amp;nbsp; 37oC,4h&lt;br&gt; <br /> | rowspan=&quot;2&quot; | <br /> Ni beads &lt;br&gt; <br /> <br /> | rowspan=&quot;2&quot; | &lt;br&gt; 7.00 / 38090.41 &lt;br&gt; <br /> | &amp;nbsp; &lt;br&gt; low&lt;br&gt;<br /> |-<br /> | 4&lt;br&gt; <br /> | <br /> 0.2 mM IPTG&amp;nbsp;&amp;nbsp; RT o/n &amp;nbsp; <br /> <br /> | yes&lt;br&gt;<br /> |-<br /> | 5&lt;br&gt; <br /> | pCold-FN3K&lt;br&gt; <br /> | 1829&lt;br&gt; <br /> | <br /> &amp;nbsp;5 ml, <br /> <br /> BL21DE3pRare2pLysS &lt;br&gt; <br /> <br /> | 0.2 mM IPTG&amp;nbsp; 15oC,o/n&lt;br&gt; <br /> | Ni beads&lt;br&gt; <br /> | <br /> 7.29 / 38063.47 &lt;br&gt; <br /> <br /> | &lt;br&gt;<br /> |-<br /> | 6&lt;br&gt; <br /> | rowspan=&quot;2&quot; | pET28b-FN3K&lt;br&gt; <br /> | rowspan=&quot;2&quot; | 1830&lt;br&gt; <br /> | rowspan=&quot;2&quot; | &amp;nbsp;&amp;nbsp; 5 ml, <br /> BL21DE3pRare2pLysS &lt;br&gt; &lt;br&gt; <br /> <br /> | <br /> 0.2 mM IPTG&amp;nbsp;&amp;nbsp; 37oC,4h&lt;br&gt; <br /> <br /> | rowspan=&quot;2&quot; | Ni beads &lt;br&gt; <br /> | rowspan=&quot;2&quot; | 7.29 / 38794.21 &lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 7&lt;br&gt; <br /> | <br /> 0.2 mM IPTG&amp;nbsp;&amp;nbsp; RT o/n&lt;br&gt; <br /> <br /> | &lt;br&gt;<br /> |-<br /> | 8&lt;br&gt; <br /> | rowspan=&quot;2&quot; | pET30b-FN3K&lt;br&gt; <br /> | rowspan=&quot;2&quot; | &lt;br&gt; 1831&lt;br&gt; <br /> | rowspan=&quot;2&quot; | &amp;nbsp; 5 ml, <br /> BL21DE3pRare2pLysS &lt;br&gt; <br /> <br /> | <br /> 0.2 mM IPTG&amp;nbsp;&amp;nbsp; 37oC,4h&lt;br&gt; <br /> <br /> | &lt;br&gt; <br /> | rowspan=&quot;2&quot; | &lt;br&gt;&amp;nbsp; 6.95 / 36349.49 &lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 9&lt;br&gt; <br /> | <br /> 0.2 mM IPTG&amp;nbsp;&amp;nbsp;&amp;nbsp; RT o/n&lt;br&gt; <br /> <br /> | Ni beads&lt;br&gt; <br /> | low&lt;br&gt;<br /> |-<br /> | 10&lt;br&gt; <br /> | PageRuler Plus <br /> protein ladder &lt;br&gt; <br /> <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &amp;nbsp; &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 11&lt;br&gt; <br /> | rowspan=&quot;2&quot; | pCold-HTNCre&lt;br&gt; <br /> | rowspan=&quot;2&quot; | HTNCre&lt;br&gt; <br /> | rowspan=&quot;2&quot; | 1823&lt;br&gt; <br /> | rowspan=&quot;1&quot; | &lt;br&gt; 5 ml, <br /> Nico21(DE3)plysN&lt;br&gt; <br /> <br /> | rowspan=&quot;2&quot; | <br /> 0.2 mM IPTG 15oc o/n &lt;br&gt; <br /> <br /> | Ni beads&lt;br&gt; <br /> | rowspan=&quot;2&quot; | &amp;nbsp; &lt;br&gt; 9.47 / 45756.94 &lt;br&gt; <br /> | &amp;nbsp; YES&lt;br&gt;<br /> |-<br /> | 12&lt;br&gt; <br /> | rowspan=&quot;1&quot; | <br /> &lt;br&gt; ] <br /> <br /> | Ni column&lt;br&gt; <br /> | YES&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Protein_Purification]]</div> Paul.xu