https://eln.excellgen.com/index.php?action=history&feed=atom&title=1833-pT7A3-TRX 1833-pT7A3-TRX - Revision history 2026-10-18T14:54:58Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1833-pT7A3-TRX&diff=10152&oldid=prev Paul.xu: Created page with "== '''Cloning of 1833-pT7A3-TRX''' == '''Purpose:''' Cloning of 1833-pT7A3-TRX '''Date: '''2013-8-25---2013-8-29 '''Notebook:0''' '''Page:''' '''Clone ID:1833''' '''Exp..." 2013-08-30T13:52:09Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 1833-pT7A3-TRX&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1833-pT7A3-TRX &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-8-25---2013-8-29 &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clone ID:1833&#039;&#039;&#039; &#039;&#039;&#039;Exp...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1833-pT7A3-TRX&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1833-pT7A3-TRX<br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-8-25---2013-8-29<br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1833&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1833PCR_8-27-2013.JPG]]&lt;br&gt;<br /> <br /> PCR from BL21(DE3) genomic DNA&lt;br&gt;TRX-Nco1-F&lt;br&gt;TRX-Nde1-R&lt;br&gt;<br /> <br /> [[Image:1826+1833clone_8-28-2013.JPG]]&lt;br&gt;<br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; height=&quot;86&quot; width=&quot;374&quot;<br /> |-<br /> | lane&lt;br&gt;<br /> | 3&lt;br&gt;<br /> | 4&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | PCR product&lt;br&gt;<br /> | 1279,pT7A3&lt;br&gt;<br /> |-<br /> | enzymes&lt;br&gt;<br /> | colspan=&quot;2&quot; | &amp;nbsp; Nco1 + Nde1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt;<br /> <br /> <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-8-29<br /> <br /> Make miniprep and digest with Nco1 + Xho1 <br /> <br /> [[Image:1833digest_8-29-2013.JPG]]&lt;br&gt; <br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; [[Category:Cloning]]</div> Paul.xu