https://eln.excellgen.com/index.php?action=history&feed=atom&title=1836_pCold-MBP-Taq-Muts 1836 pCold-MBP-Taq-Muts - Revision history 2026-10-18T17:16:30Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1836_pCold-MBP-Taq-Muts&diff=10331&oldid=prev Paul.xu at 20:26, 26 September 2013 2013-09-26T20:26:19Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1836 pCold-MBP-Taq-Muts&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of1836 pCold-MBP-Taq-Muts <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-8-30-- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1836&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; Shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> PCR from 352 pET3a-Muts &lt;br&gt; <br /> <br /> Taq-Muts-F CAT ACA TAT GGA AGG CAT GCT GAA GGG C &lt;br&gt; <br /> <br /> Taq-Muts-HindIII-R tgctagAAGCTTCCCCTTCATGGTATCCAAGG <br /> <br /> [[Image:1836pcr.JPG]]&lt;br&gt; digest with Nde1 + HindIII,&lt;br&gt; <br /> <br /> [[Image:Digest for 1836.JPG]]&lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-8-30 <br /> <br /> Make miniprep and digest with HindIII+BamHI <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Cloning of 1836 pCold-MBP-Taq-Muts&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of1836 pCold-MBP-Taq-Muts <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-8-30-- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1836&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; Shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> PCR from 352 pET3a-Muts &lt;br&gt; <br /> <br /> Taq-Muts-F CAT ACA TAT GGA AGG CAT GCT GAA GGG C &lt;br&gt; <br /> <br /> Taq-Muts-HindIII-R tgctagAAGCTTCCCCTTCATGGTATCCAAGG <br /> <br /> [[Image:1836pcrq5.JPG]]&lt;br&gt; digest with Nde1 + HindIII,&lt;br&gt; <br /> <br /> [[Image:Digest for 1836 367fix2.JPG]]&lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-8-30 <br /> <br /> Make miniprep and digest with &lt;br&gt; <br /> <br /> [[Image:1836DIGEST.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Paul.xu