https://eln.excellgen.com/index.php?action=history&feed=atom&title=1837_pPink%CE%B1-HC-Enterokinase 1837 pPinkα-HC-Enterokinase - Revision history 2026-10-18T22:24:19Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1837_pPink%CE%B1-HC-Enterokinase&diff=10579&oldid=prev Paul.xu at 17:36, 4 November 2013 2013-11-04T17:36:13Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1837 pPinkα-HC-Enterokinase&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of1837 pPinkα-HC-Enterokinase <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-8-30-- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1837&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; Shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> PCR from 1815 pMAL-c5E-Enterokinase&lt;br&gt; <br /> <br /> [[Image:1837PCR.JPG]] <br /> <br /> digest with FspI + Kpn1 <br /> <br /> ligate to StuI + Kpn1 digested vector pPinkα-HC <br /> <br /> [[Image:DIGEST FOR1837.JPG]]&lt;br&gt;&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-8-30 <br /> <br /> Make miniprep and digest with NdeI+KpnI---&amp;gt;675bp&lt;br&gt; <br /> <br /> [[Image:1837DIGEST.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> 2013-9-3&lt;br&gt; <br /> <br /> digest with Pst1+Kpn1, should be 300bp <br /> <br /> [[Image:1837digest 9-3-2013.JPG]] <br /> <br /> == &#039;&#039;&#039;Cloning of 1837 pPinkα-HC-Enterokinase&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of1837 pPinkα-HC-Enterokinase <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-10-30-- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1837&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; Shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> PCR from 1815 pMAL-c5E-Enterokinase&lt;br&gt; <br /> <br /> Enterokinase-Xho1-F2 tagctactcgagaaa agg attgtcggaggaagtgactc&lt;br&gt;Enterokinase-Kpn1-R tgctagGGTACCCTAgTGATGATGATGATGATGTAGAAAAC&lt;br&gt;[[Image:1837pcr.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> digest with XhoI + Kpn1 <br /> <br /> ligate to XhoI + Kpn1 digested vector pPinkα-HC <br /> <br /> [[Image:Digest for 1837 1918 1919.JPG]]&lt;br&gt;&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-11-4 <br /> <br /> Make miniprep and digest with XhoI+KpnI&lt;br&gt; <br /> <br /> [[Image:1837_1892digest.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Paul.xu