https://eln.excellgen.com/index.php?action=history&feed=atom&title=1840-pT7A3-FluA-NP 2026-10-18T21:25:54Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1840-pT7A3-FluA-NP&diff=10198&oldid=prev 2013-09-06T15:34:11Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1840-pT7A3-FluA-NP&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1840-pT7A3-FluA-NP <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-9-4---2013-9-5 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1840&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> PCR the same with 1826<br /> <br /> [[Image:1840clone_9-4-2013.JPG]]<br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; height=&quot;86&quot; width=&quot;367&quot;<br /> |-<br /> | lane<br /> | 1<br /> | 2<br /> |-<br /> | <br /> | PCR product<br /> | 1505,pT7A3-endo1<br /> |-<br /> | enzymes<br /> | colspan=&quot;2&quot; | &amp;nbsp;&amp;nbsp;&amp;nbsp; Nde1+Xho1&lt;br&gt;<br /> |}<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-9-5<br /> <br /> Make miniprep and digest with Xba1 + Xho1 <br /> <br /> [[Image:1840digest_9-5-2013.JPG]]&lt;br&gt; <br /> <br /> clone 1,3,5 may be right, need to sequence<br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div>

Paul.xu