https://eln.excellgen.com/index.php?action=history&feed=atom&title=1841-pCMV5-PKAalpha 1841-pCMV5-PKAalpha - Revision history 2026-10-18T10:58:13Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1841-pCMV5-PKAalpha&diff=11655&oldid=prev Paul.xu at 19:41, 9 April 2014 2014-04-09T19:41:26Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2183-pEC-PKAalpha&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2183-pEC-PKAalpha <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-4-1--2014-4-3 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2183&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:731 1841PCR 3-26-2014.JPG]] <br /> <br /> [[Image:1841 2128clone 4-1-2014.JPG]]&lt;br&gt; <br /> <br /> {| width=&quot;310&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt; <br /> | 1&lt;br&gt; <br /> | 2&lt;br&gt; <br /> | 3&lt;br&gt; <br /> | 4&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | pcr&lt;br&gt; <br /> | 1395, pCMV5-hIgG-Lc-TagGFP-Fc(3c)-WPRE-OriP&lt;br&gt; <br /> | pcr&lt;br&gt; <br /> | 1454,pEC&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | colspan=&quot;3&quot; | &amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp; Nhe1+Kpn1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014-4-3 <br /> <br /> Make miniprep and digest with Xho1&lt;br&gt; <br /> <br /> [[Image:1841digest 4-3-2014.JPG]]&lt;br&gt; <br /> <br /> 6~10:from vector 1454,pEC&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Cloning of 1841-pCMV5-PKAalpha&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1841-pCMV5-PKAalpha<br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-4-6---2014-4-9<br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1841&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:732_1841_2145_2147clone_4-7-2014.JPG]]&lt;br&gt; <br /> <br /> {| width=&quot;200&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt;<br /> | 3&lt;br&gt;<br /> | 4&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | 2183pEC-PKAalpha &lt;br&gt;<br /> | 13951395 pCMV5-hIgG-Lc-TagGFP-Fc(3c)-WPRE-OriP&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | colspan=&quot;2&quot; | Nhe1+Kpn1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt;<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014-4-9<br /> <br /> Make miniprep and digest with Xho1 <br /> <br /> [[Image:732_1841digest_4-9-2014.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Paul.xu