https://eln.excellgen.com/index.php?action=history&feed=atom&title=1846-pT7A3-6H-sso7d 2026-10-18T21:32:39Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1846-pT7A3-6H-sso7d&diff=10268&oldid=prev 2013-09-19T19:42:13Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1846-pT7A3-6H-sso7d&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1846-pT7A3-6H-sso7d <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-9-16---2013-9-19 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1846&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:673pcr+1845pcr+1846PCR_9-16-2013.JPG]]&lt;br&gt;<br /> <br /> PCR sso7d using&amp;nbsp; 1734 pT7A30-sso7d-KX&lt;br&gt;sso7d-Nco1-F &lt;br&gt;sso7d-Nde1-R&lt;br&gt;<br /> <br /> [[Image:1846+1866clone_9-17-2013.JPG]]&lt;br&gt;<br /> <br /> {| width=&quot;358&quot; height=&quot;86&quot; border=&quot;1&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt;<br /> | 1&lt;br&gt;<br /> | 2&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | PCR product&lt;br&gt;<br /> | 1279,pT7A3&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | colspan=&quot;2&quot; | &amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp; Nco1 + Nde1 &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt;<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-9-19 <br /> <br /> Make miniprep and digest with Nco1 + Xho1 <br /> <br /> [[Image:1846digest_9-19-2013.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div>

Paul.xu