https://eln.excellgen.com/index.php?action=history&feed=atom&title=1847-pT7A30-sso7d-6H 2026-10-18T21:32:43Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1847-pT7A30-sso7d-6H&diff=10285&oldid=prev 2013-09-23T15:02:28Z

<p></p> <p><b>New page</b></p><div>= &#039;&#039;&#039;Cloning of &#039;&#039;&#039;1847-pT7A30-sso7d-6H =<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1847-pT7A30-sso7d-6H <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-9-17---2013-9-20 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1847&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1847+1848PCR_9-17-2013.JPG]]&lt;br&gt;<br /> <br /> PCR using 1734 pT7A30-sso7d-KX&amp;nbsp; as template &lt;br&gt;Sso7d-Not1-F&lt;br&gt;sso7d-Sal1-R&lt;br&gt;<br /> <br /> [[Image:1845+1847+1848clone 9-18-2013.JPG]] <br /> <br /> {| width=&quot;353&quot; height=&quot;103&quot; border=&quot;1&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt;<br /> | 3&lt;br&gt;<br /> | 4&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | PCR product&lt;br&gt;<br /> | 1727 pT7A30&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | Not1 + Sal1&lt;br&gt;<br /> | Not1 + Xho1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt;<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-9-20<br /> <br /> Make miniprep and digest with Kpn1&lt;br&gt;<br /> <br /> [[Image:1847+1848digest_9-20-2013.JPG]]<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div>

Paul.xu