https://eln.excellgen.com/index.php?action=history&feed=atom&title=1848-pJ912-Enterokinase 1848-pJ912-Enterokinase - Revision history 2026-10-18T17:23:02Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1848-pJ912-Enterokinase&diff=10286&oldid=prev Paul.xu: Created page with "= '''Cloning of '''1848-pJ912-Enterokinase = '''Purpose:''' Cloning of 1848-pJ912-Enterokinase '''Date: '''2013-9-17---2013-9-20 '''Notebook:0''' '''Page:''' '''Clone ID:1..." 2013-09-23T15:05:47Z <p>Created page with &quot;= &#039;&#039;&#039;Cloning of &#039;&#039;&#039;1848-pJ912-Enterokinase = &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1848-pJ912-Enterokinase &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-9-17---2013-9-20 &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clone ID:1...&quot;</p> <p><b>New page</b></p><div>= &#039;&#039;&#039;Cloning of &#039;&#039;&#039;1848-pJ912-Enterokinase =<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1848-pJ912-Enterokinase<br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-9-17---2013-9-20<br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1848&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1847+1848PCR_9-17-2013.JPG]]&lt;br&gt;<br /> <br /> PCR from 1731 pT7A3-TurboEnterokinase&lt;br&gt;Enterokinase-Xho1-F&lt;br&gt;Enterokinase-Not1-R&lt;br&gt;<br /> <br /> [[Image:1845+1847+1848clone_9-18-2013.JPG]]&lt;br&gt;<br /> <br /> {| width=&quot;374&quot; height=&quot;86&quot; border=&quot;1&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt;<br /> | 5&lt;br&gt;<br /> | 6&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | PCR product&lt;br&gt;<br /> | 1842 pJ912-Irisin-1&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | colspan=&quot;2&quot; | Xho1 + Eag1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt;<br /> <br /> <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-9-20<br /> <br /> Make miniprep and digest with Xho1 + Eag1&lt;br&gt;<br /> <br /> [[Image:1847+1848digest_9-20-2013.JPG]]<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; [[Category:Cloning]]</div> Paul.xu