https://eln.excellgen.com/index.php?action=history&feed=atom&title=1849_1813_1811_1824_1825 1849 1813 1811 1824 1825 - Revision history 2026-10-18T08:21:06Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1849_1813_1811_1824_1825&diff=10291&oldid=prev Paul.xu at 16:38, 23 September 2013 2013-09-23T16:38:29Z <p></p> <p><b>New page</b></p><div>Date: 2013-9-20<br /> <br /> FN3K and FLP purification<br /> <br /> [[Image:2013 9 20.jpg]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> {| width=&quot;200&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt; <br /> | 1&lt;br&gt; <br /> | 2&lt;br&gt; <br /> | 3&lt;br&gt; <br /> | 4&lt;br&gt; <br /> | 5&lt;br&gt; <br /> | 6&lt;br&gt; <br /> | 7&lt;br&gt; <br /> | 8&lt;br&gt; <br /> | 9&lt;br&gt; <br /> | M&lt;br&gt; <br /> | 10&lt;br&gt; <br /> | 11&lt;br&gt;<br /> |-<br /> | Clone ID&lt;br&gt; <br /> | 1849&lt;br&gt; <br /> | 1849&lt;br&gt; <br /> | 1813&lt;br&gt; <br /> | 1813&lt;br&gt; <br /> | 1813&lt;br&gt; <br /> | 1813&lt;br&gt; <br /> | 1813&lt;br&gt; <br /> | 1811&lt;br&gt; <br /> | 1811&lt;br&gt; <br /> | &lt;br&gt; <br /> | 1824&lt;br&gt; <br /> | 1825&lt;br&gt;<br /> |-<br /> | Name&lt;br&gt; <br /> | FN3K&lt;br&gt; <br /> | FN3K&lt;br&gt; <br /> | HTNFlp-Ecoli&lt;br&gt; <br /> | HTNFlp-Ecoli&lt;br&gt; <br /> | HTNFlp-Ecoli&lt;br&gt; <br /> | HTNFlp-Ecoli&lt;br&gt; <br /> | HTNFlp-Ecoli&lt;br&gt; <br /> | HTNFlp-Ecoli&lt;br&gt; <br /> | pT7A3-VPol1&lt;br&gt; <br /> | Fermentas PageRuler Plus&lt;br&gt; <br /> | pCold-MBP-(a3C)-NRF2-3C&lt;br&gt; <br /> | pCold-MBP-(a3C)-NRF2-pLacZ-3C&lt;br&gt;<br /> |-<br /> | Desc&lt;br&gt; <br /> | &#039;&#039;&#039;50&#039;&#039;&#039; mM NaCl, 300 mM Imidazole elute from Ni column&lt;br&gt; <br /> | &#039;&#039;&#039;500&#039;&#039;&#039; mM NaCl, 300 mMImidazole elute from Ni column&lt;br&gt; <br /> | &amp;nbsp;Q column flow through&lt;br&gt; <br /> | Q column eluted with 1 M NaCl&lt;br&gt; <br /> | SP flow through&lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | elute from Ni column&lt;br&gt; <br /> | &lt;br&gt; <br /> | small scall expression screening&lt;br&gt; <br /> | small scall expression screening&lt;br&gt;<br /> |-<br /> | Sample treatment &lt;br&gt; <br /> | load sample to Ni column, wash with Buffer A&lt;br&gt; <br /> | load sample to Ni column, wash with Buffer A&lt;br&gt; <br /> | concentrate Ni resin purified protein by spin column, buffer exchange to adjust salt conc to 50 mM NaCl, load to Q column&lt;br&gt; <br /> | lconcentrate Ni resin purified protein by spin column, buffer exchange to adjust salt conc to 50 mM NaCl, load to Q column&lt;br&gt; <br /> | concentrate Ni resin purified protein by spin column, buffer exchange to adjust salt conc to 50 mM NaCl, load to SP column&lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | load sample to Ni column, wash with Buffer A&lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Protein_Purification]]</div> Paul.xu