https://eln.excellgen.com/index.php?action=history&feed=atom&title=1850_pJ912-Monellin-Y 1850 pJ912-Monellin-Y - Revision history 2026-10-18T19:41:28Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1850_pJ912-Monellin-Y&diff=10327&oldid=prev Paul.xu at 18:45, 26 September 2013 2013-09-26T18:45:19Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1850 pJ912-Monellin-Y&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of1850 pJ912-Monellin-Y <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-9-23-- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1850&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; Shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> PCR from 655 <br /> <br /> Monellin-Xho1-F <br /> <br /> Monellin-Not1-R <br /> <br /> [[Image:1850pcr.JPG]]&lt;br&gt; <br /> <br /> use Xho1 + Eag1 for cloning <br /> <br /> [[Image:Digest_for1850.JPG]]&lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-8-30 <br /> <br /> Make miniprep and digest with Xho1 + Eag1 <br /> <br /> [[Image:1850digest.JPG]]<br /> <br /> &lt;br&gt;<br /> <br /> [[Category:Cloning]]</div> Paul.xu