https://eln.excellgen.com/index.php?action=history&feed=atom&title=1853-pET28b-yFLP 1853-pET28b-yFLP - Revision history 2026-10-18T16:41:01Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1853-pET28b-yFLP&diff=10436&oldid=prev Paul.xu: Created page with "== '''Cloning of 1853-pET28b-yFLP''' == '''Purpose:''' Cloning of 1853-pET28b-yFLP '''Date: '''2013-10-16---2013-10-18 '''Notebook:0''' '''Page:''' '''Clone ID:1853''' ..." 2013-10-21T15:07:28Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 1853-pET28b-yFLP&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1853-pET28b-yFLP &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-10-16---2013-10-18 &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clone ID:1853&#039;&#039;&#039; ...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1853-pET28b-yFLP&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1853-pET28b-yFLP<br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-10-16---2013-10-18<br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1853&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1853pcr_10-15-2013.JPG]]&lt;br&gt; <br /> <br /> PCR using 1857 pOG44 as template, <br /> <br /> &lt;br&gt;pcr 1:<br /> <br /> FLP-HindIII-F&lt;br&gt;FLP-L33S-R&lt;br&gt;&lt;br&gt;<br /> <br /> pcr 2:&lt;br&gt;FLP-L33S-F&lt;br&gt;FLP-Y108N-R&lt;br&gt;&lt;br&gt;<br /> <br /> &lt;br&gt;pcr 3:&lt;br&gt;FLP-Y108N-F&lt;br&gt;FLP-S294P-R&lt;br&gt;&lt;br&gt;<br /> <br /> pcr 4:&lt;br&gt;FLP-S294P-F&lt;br&gt;FLP-Xho1-R&lt;br&gt;<br /> <br /> [[Image:1853+1870PCR_10-16-2-13.JPG]]&lt;br&gt;<br /> <br /> Join 4 fragments using &lt;br&gt;FLP-HindIII-F + FLP-Xho1-R&lt;br&gt;<br /> <br /> [[Image:1853+1869+1870clone_10-16-2013.JPG]]&lt;br&gt;<br /> <br /> {| width=&quot;413&quot; height=&quot;82&quot; border=&quot;1&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt;<br /> | 1&lt;br&gt;<br /> | 2&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | PCR &lt;br&gt;<br /> | 375, pET28b-Cre&lt;br&gt;&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | colspan=&quot;2&quot; | HindIII + Xho1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt;<br /> <br /> <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-10-18<br /> <br /> Make miniprep and digest with&amp;nbsp; Hind111+EcoR1 <br /> <br /> [[Image:1853digest_10-18-2013.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Paul.xu