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1854-pPinkalpha-HC-yFLP - Revision history
2026-10-18T19:04:10Z
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Paul.xu at 14:13, 1 November 2013
2013-11-01T14:13:04Z
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<p><b>New page</b></p><div>== '''Cloning of 1854-pPinkalpha-HC-yFLP''' ==<br />
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'''Purpose:''' Cloning of 1854-pPinkalpha-HC-yFLP <br />
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'''Date: '''2013-10-28---2013-10-31 <br />
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'''Notebook:0''' <br />
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'''Page:''' <br />
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'''Clone ID:1854''' <br />
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'''Experimenters:''' yingying <br />
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== '''Results''' ==<br />
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=== cloning ===<br />
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[[Image:1854_1869_1899PCR_10-29-2013.JPG]]<br><br />
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PCR using 1853 pET28b-yFLP as template<br><br />
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6XHis-Xho1-F2<br>Flp-Kpn1-R<br><br />
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[[Image:1854_1869_1889_1899clone_10-29-2013.JPG]][[Image:1854_1899vector_10-29-2013.JPG]]<br><br />
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{| width="291" height="82" border="1" cellspacing="1" cellpadding="1"<br />
|-<br />
| lane<br><br />
| 1<br><br />
| 2<br><br />
|-<br />
| <br><br />
| PCR product<br><br />
| 215 pPinkalpha-HC<br><br />
|-<br />
| <br><br />
| colspan="2" | Xho1 + Kpn1<br><br />
|}<br />
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=== Colony Screening ===<br />
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Date: 2013-10-31<br />
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Make miniprep and digest with Xho1 + Kpn1/Hind111<br />
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[[Image:1854digest_10-31-2013.JPG]][[Image:1854+1899digest_10-31-2013.JPG]]<br><br />
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== '''Result intepretation/Summary''' ==<br />
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== '''Conclusion''' ==<br />
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<br> <br />
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[[Category:Cloning]]</div>
Paul.xu