https://eln.excellgen.com/index.php?action=history&feed=atom&title=1860-pT7A3-yFLP 1860-pT7A3-yFLP - Revision history 2026-10-18T16:40:59Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1860-pT7A3-yFLP&diff=10464&oldid=prev Paul.xu: Created page with "== '''Cloning of 1860-pT7A3-yFLP''' == '''Purpose:''' Cloning of 1860-pT7A3-yFLP '''Date: '''2013-10-21----2013-10-22 '''Notebook:0''' '''Page:''' '''Clone ID:1860''' '..." 2013-10-23T14:50:14Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 1860-pT7A3-yFLP&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1860-pT7A3-yFLP &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-10-21----2013-10-22 &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clone ID:1860&#039;&#039;&#039; &#039;...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1860-pT7A3-yFLP&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1860-pT7A3-yFLP<br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-10-21----2013-10-22<br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1860&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1860_1884_1869clone_10-21-2013.JPG]]<br /> <br /> {| width=&quot;299&quot; height=&quot;82&quot; border=&quot;1&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt;<br /> | 1&lt;br&gt;<br /> | 2&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | 1853 pET28b-yFLP&lt;br&gt;<br /> | 1279 pT7A3&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | colspan=&quot;2&quot; | NcoI+Xho1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt;<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-10-22 <br /> <br /> Make miniprep and digest with NcoI+Xho1&lt;br&gt;<br /> <br /> [[Image:1860digest_10-22-2013.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; [[Category:Cloning]]</div> Paul.xu