https://eln.excellgen.com/index.php?action=history&feed=atom&title=1865_pT7A3-MBP-FluA-NP 1865 pT7A3-MBP-FluA-NP - Revision history 2026-10-18T21:27:56Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1865_pT7A3-MBP-FluA-NP&diff=10337&oldid=prev Paul.xu at 21:41, 26 September 2013 2013-09-26T21:41:16Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1865 pT7A3-MBP-FluA-NP&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of1865 pT7A3-MBP-FluA-NP <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-9-26-- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1865&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; Shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> subclone MBP from 1804 pCold-MBP-(E3C) to 1844 pT7A3-His-MBP-(E3C)-FluA-NP using Nco1 + Nde1 <br /> <br /> [[Image:Digest for1865.JPG]] <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-8-30 <br /> <br /> Make miniprep and digest with Nco1 + Nde1 &lt;br&gt; <br /> <br /> [[Image:1865DIGEST.JPG]][[Image:1865DIGEST3.JPG]]&lt;br&gt; digest with Xho1 + Nde1 <br /> <br /> [[Image:1865DIGEST2.JPG]]<br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Paul.xu