https://eln.excellgen.com/index.php?action=history&feed=atom&title=1866-pET28b-Enterokinase 1866-pET28b-Enterokinase - Revision history 2026-10-18T23:55:21Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1866-pET28b-Enterokinase&diff=10264&oldid=prev Paul.xu at 17:46, 19 September 2013 2013-09-19T17:46:42Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1866-pET28b-Enterokinase&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1866-pET28b-Enterokinase <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-9-16---2013-9-19 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1866&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1846+1866clone 9-17-2013.JPG]]&lt;br&gt; <br /> <br /> {| width=&quot;399&quot; border=&quot;1&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt; <br /> | 3&lt;br&gt; <br /> | 4&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | 1731,pT7A3-Enterokinase&lt;br&gt; <br /> | 1793,pET28b-VPol1&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | colspan=&quot;2&quot; | &amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp; NcoI +Xho1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-9-19 <br /> <br /> Make miniprep and digest with&amp;nbsp; NcoI +Xho1 <br /> <br /> [[Image:1866digest_9-19-2013.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div> Paul.xu