https://eln.excellgen.com/index.php?action=history&feed=atom&title=1877_pFastBac-MMV_VP2_Strep_II 2026-10-18T18:04:43Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1877_pFastBac-MMV_VP2_Strep_II&diff=10677&oldid=prev 2013-11-25T13:26:13Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1877 pFastBac-MMV VP2 Strep II&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1877 pFastBac-MMV VP2 Strep II <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-11-6--- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1877&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === SUBCLONING ===<br /> <br /> subcloning using EcoR1 + HindIII from 1876 to 49 pFastBac1 <br /> <br /> [[Image:DIGEST FOR 1875 1877.JPG]]&lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-11-8 <br /> <br /> Make miniprep, digest with EcoR1 + HindIII <br /> <br /> [[Image:1875 1877digest.JPG]] <br /> <br /> &lt;br&gt; Analyzing Recombinant Bacmid DNA by PCR&lt;br&gt;Use these two primers to do PCR&lt;br&gt;M13-F CCCAGTCACGACGTTGTAAAACG&lt;br&gt;M13-R AGCGGATAACAATTTCACACAGG &lt;br&gt; <br /> <br /> PCR product should be~5000bp. If it is only ~300 bp, then is is empty vector. &lt;br&gt; <br /> <br /> [[Image:1875 1877indh10bac.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div>

Paul.xu