https://eln.excellgen.com/index.php?action=history&feed=atom&title=1879-pLysN-PampR-mNhe1 1879-pLysN-PampR-mNhe1 - Revision history 2026-10-18T21:01:39Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1879-pLysN-PampR-mNhe1&diff=10528&oldid=prev Paul.xu at 20:33, 28 October 2013 2013-10-28T20:33:05Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1879-pLysN-PampR-mNhe1&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1879-pLysN-PampR-mNhe1 <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-10-25---2013-10-28 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1879&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1879_1882_1883_1888_1889PCR_10-22-2013.JPG]]&lt;br&gt;<br /> <br /> PCR AmpR promoter using pUC19 plasmid&lt;br&gt;AmpR-pro-HindIII-F &lt;br&gt;AmpR-pro-Nde1-R&lt;br&gt;<br /> <br /> [[Image:1879+1883clone_10-24-2013.JPG]]&lt;br&gt;<br /> <br /> {| width=&quot;334&quot; height=&quot;99&quot; border=&quot;1&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt;<br /> | 1&lt;br&gt;<br /> | 2&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | PCR product&lt;br&gt;<br /> | 674 pLysN-mNhe1&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | colspan=&quot;2&quot; | &amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp; HindIII + Nde1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt;<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-10-28 <br /> <br /> Make miniprep and digest with HindIII + Nde1<br /> <br /> [[Image:1879+1883digest_10-28-2013.JPG]]&lt;br&gt;<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div> Paul.xu