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1884-pT7A30HTNFlp-ecoli - Revision history
2026-10-18T21:27:59Z
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Paul.xu at 15:20, 24 January 2014
2014-01-24T15:20:35Z
<p></p>
<p><b>New page</b></p><div>== '''Cloning of 1884-pT7A30HTNFlp-ecoli''' ==<br />
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'''Purpose:''' Cloning of 1884-pT7A30HTNFlp-ecoli <br />
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'''Date: '''2013-10-22---2013-10-24 <br />
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'''Notebook:0''' <br />
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'''Page:''' <br />
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'''Clone ID:1884''' <br />
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'''Experimenters:''' yingying <br />
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== '''Results''' ==<br />
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=== cloning ===<br />
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[[Image:1884clone 10-22-2013.JPG]]<br> <br />
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{| width="260" cellspacing="1" cellpadding="1" border="1"<br />
|-<br />
| lane<br> <br />
| 1<br> <br />
| 2<br><br />
|-<br />
| <br> <br />
| 1870 pET28b-HTNFlp-ecoli<br> <br />
| 1279 pT7A3<br><br />
|-<br />
| <br> <br />
| colspan="2" | &nbsp;&nbsp; Nco1+ Xho1<br><br />
|}<br />
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<br> <br />
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=== Colony Screening ===<br />
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Date: 2013-10-23 <br />
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Make miniprep and digest with Nco1+ Xho1 <br />
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[[Image:1884digest 10-23-2013.JPG]]<br> <br />
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Date: 2013-10-24 <br />
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Make miniprep and digest with Hind111+ Xho1 <br> <br />
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[[Image:1884digest 10-24-2013.JPG]] <br />
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clone3 right<br> <br />
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<br><br />
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2014-1-23<br> <br />
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digest with EcoR1+ Xho1 <br><br />
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[[Image:1884digest_1-23-2014.JPG]]<br />
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== '''Result intepretation/Summary''' ==<br />
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== '''Conclusion''' ==<br />
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<br> <br />
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[[Category:Cloning]]</div>
Paul.xu