https://eln.excellgen.com/index.php?action=history&feed=atom&title=1885_pT7A3-HC 2026-10-18T21:23:55Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1885_pT7A3-HC&diff=10500&oldid=prev 2013-10-25T20:18:39Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1885 pT7A3-HC&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1885 pT7A3-HC <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-10-21--- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1885&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> digest pRsetA with DraIII + Sap1---&amp;gt; 2145 bp fragment (ampR + pUC orig), top band&lt;br&gt; <br /> <br /> Digest pT7A3 with DraIII + Sap1--&amp;gt;3412 bp fragment (promoter, MCS, LacI), top band <br /> <br /> [[Image:Digest for1885.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-10-23 <br /> <br /> grow on Amp, but won&#039;t grow on Kan,&lt;br&gt;<br /> <br /> Make miniprep, digest with EcoRV + EcoR1 should have 2 fragments: the small one should be ~ 1381 bp fragment&lt;br&gt; <br /> <br /> [[Image:1885DIGEST.JPG]] <br /> <br /> STOCK1885-1&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div>

Paul.xu