https://eln.excellgen.com/index.php?action=history&feed=atom&title=1890-pCMV5-MMLV-gag-NoStop-WPRE-oriP 1890-pCMV5-MMLV-gag-NoStop-WPRE-oriP - Revision history 2026-10-18T21:32:56Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1890-pCMV5-MMLV-gag-NoStop-WPRE-oriP&diff=10486&oldid=prev Paul.xu at 18:56, 25 October 2013 2013-10-25T18:56:06Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1890-pCMV5-MMLV-gag-NoStop-WPRE-oriP&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1890-pCMV5-MMLV-gag-NoStop-WPRE-oriP <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-10-23---2013-10-25 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1890&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1878_1890PCR_10-23-2013.JPG]]&lt;br&gt;<br /> <br /> PCR from 1713 MMLV&lt;br&gt;MMLV-Gag-BamH1-F&lt;br&gt;MMLV-Gag-EcoR1-R&lt;br&gt;<br /> <br /> [[Image:1879 1888 1890clone 10-23-2013.JPG]] <br /> <br /> {| width=&quot;380&quot; border=&quot;1&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | PCR product&lt;br&gt; <br /> | 1449,pCMV5-Gag-NoStop-WPRE-oriP&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | colspan=&quot;2&quot; | &amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp; BamH1 + EcoR1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-10-25 <br /> <br /> Make miniprep and digest with BamH1 + EcoR1/ BamH1 + sph1&lt;br&gt; <br /> <br /> c: 1449pCMV5-Gag-NoStop-WPRE-oriP&lt;br&gt; <br /> <br /> [[Image:1890digest 10-25-2013.JPG]]&lt;br&gt; <br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div> Paul.xu