https://eln.excellgen.com/index.php?action=history&feed=atom&title=1891-pET28b-TAB5 1891-pET28b-TAB5 - Revision history 2026-10-18T11:59:57Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1891-pET28b-TAB5&diff=10621&oldid=prev Paul.xu at 14:38, 15 November 2013 2013-11-15T14:38:42Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1891-pET28b-TAB5&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1891-pET28b-TAB5 <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-10-29----2013-11-1 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1891&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1315 1891pcr 10-30-2013.JPG]]&lt;br&gt; <br /> <br /> gene&amp;nbsp; synthesis&lt;br&gt; <br /> <br /> pcr1:TAP5-1 to TAP5-14&lt;br&gt; <br /> <br /> pcr2:TAP5-15 to TAP5-28&lt;br&gt; <br /> <br /> [[Image:1315 1891PCR 10-30-2013.JPG]]&lt;br&gt; <br /> <br /> PCR: TAP5-1 to TAP5-28&lt;br&gt; <br /> <br /> [[Image:1315 1891clone 10-30-2013.JPG]]&lt;br&gt; <br /> <br /> {| width=&quot;335&quot; border=&quot;1&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt; <br /> | 3&lt;br&gt; <br /> | 4&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | PCR product&lt;br&gt; <br /> | 1225,pET28b-T7-Endo1&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | colspan=&quot;2&quot; | &amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp; Nde1 + Xho1 &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-11-1 <br /> <br /> Make miniprep and digest with Nde1 + Xho1 <br /> <br /> [[Image:1891digest 11-1-2013.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div> Paul.xu