https://eln.excellgen.com/index.php?action=history&feed=atom&title=1892_pT7A3E 2026-10-18T19:43:25Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1892_pT7A3E&diff=10870&oldid=prev 2014-01-02T17:56:59Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1892 pT7A3E&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1892 pT7A3E <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-11-1--- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1892&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> Digest vector with Xba1 + Nde1&lt;br&gt;ligate purified vector to annealed oligos:&lt;br&gt;Xba1-rbs-Nde1-T ctaga GCTCTTTAACAATTTATCA gatcca AGGAGG atatt atg aaa atc gaa ca&lt;br&gt;Xba1-rbs-Nde1-B tatgttcgattttcataatatCCTCCTtggatcTGATAAATTGTTAAAGAGCt&lt;br&gt; <br /> <br /> [[Image:Digest_for_1892_1893.JPG]]&lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-10-28 <br /> <br /> Make miniprep, digest with NcoI+EcoRV , should GET linear the vector&lt;br&gt; <br /> <br /> [[Image:1919 1918 1892digest.JPG]] <br /> <br /> [[Image:1837 1892digest.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div>

Paul.xu