https://eln.excellgen.com/index.php?action=history&feed=atom&title=1893_pPinkalpha-HC-Monellin-Y 1893 pPinkalpha-HC-Monellin-Y - Revision history 2026-10-18T16:41:00Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1893_pPinkalpha-HC-Monellin-Y&diff=10567&oldid=prev Paul.xu: Created page with "== '''Cloning of 1893 pPinkalpha-HC-Monellin-Y''' == '''Purpose:''' Cloning of 1893 pPinkalpha-HC-Monellin-Y '''Date: '''2013-10-31--- '''Notebook:0''' '''Page:''' '''C..." 2013-11-01T19:09:28Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 1893 pPinkalpha-HC-Monellin-Y&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1893 pPinkalpha-HC-Monellin-Y &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-10-31--- &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;C...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1893 pPinkalpha-HC-Monellin-Y&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1893 pPinkalpha-HC-Monellin-Y <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-10-31--- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1893&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === PCR ===<br /> <br /> pcr using 655 as template<br /> <br /> Monellin-Kpn1-R tgctagGGTACCTTATGGTGGTGGAACTGGAC&lt;br&gt;Monellin-Xho1-F2 gatcga ctcgagaaa agg GGTGAGTGGGAGATTATTG&lt;br&gt;&lt;br&gt;[[Image:1893pcr.JPG]]&lt;br&gt; <br /> <br /> digest with KpnI+XhoI <br /> <br /> [[Image:Digest_for_1892_1893.JPG]]&lt;br&gt;&lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-11-1<br /> <br /> Make miniprep, digest with KpnI+XhoI &lt;br&gt; <br /> <br /> [[Image:1893digest.JPG‎]]&lt;br&gt; <br /> <br /> send 1893-2 to sequence&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Paul.xu