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1899-pPinkalpha-HC-HTNCre - Revision history
2026-10-18T20:32:57Z
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Paul.xu at 15:24, 1 November 2013
2013-11-01T15:24:03Z
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<p><b>New page</b></p><div>== '''Cloning of 1899-pPinkalpha-HC-HTNCre''' ==<br />
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'''Purpose:''' Cloning of 1899-pPinkalpha-HC-HTNCre <br />
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'''Date: '''2013-10-28--2013-10-31 <br />
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'''Notebook:0''' <br />
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'''Page:''' <br />
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'''Clone ID:1899''' <br />
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'''Experimenters:''' yingying <br />
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== '''Results''' ==<br />
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=== cloning ===<br />
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[[Image:1854 1869 1899PCR 10-29-2013.JPG]] <br />
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PCR using 298HTNCre as template<br>Cre-Kpn1-R <br>6XHis-Xho1-F2<br><br />
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[[Image:1854 1869 1889 1899clone 10-29-2013.JPG]][[Image:1854 1899vector 10-29-2013.JPG]] <br />
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{| width="340" height="99" border="1" cellspacing="1" cellpadding="1"<br />
|-<br />
| lane<br><br />
| 7<br><br />
| 8<br><br />
|-<br />
| <br><br />
| PCR product<br><br />
| 215 pPinkalpha-HC<br><br />
|-<br />
| <br><br />
| colspan="2" | Xho1 + Kpn1<br><br />
|}<br />
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=== Colony Screening ===<br />
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Date: 2013-10-31 <br />
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Make miniprep and digest with Xho1 + Kpn1<br />
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[[Image:1899digest 10-31-2013.JPG]] <br />
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digest with BamH1<br />
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[[Image:1854+1899digest 10-31-2013.JPG]] <br />
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== '''Result intepretation/Summary''' ==<br />
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== '''Conclusion''' ==<br />
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<br> <br />
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[[Category:Cloning]]</div>
Paul.xu