https://eln.excellgen.com/index.php?action=history&feed=atom&title=1902_pT7A3-6H-sso7d-T7-DNA-Ligase 1902 pT7A3-6H-sso7d-T7-DNA-Ligase - Revision history 2026-10-18T21:32:54Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1902_pT7A3-6H-sso7d-T7-DNA-Ligase&diff=10505&oldid=prev Paul.xu: Created page with "== '''Cloning of 1902 pT7A3-6H-sso7d-T7-DNA-Ligase''' == '''Purpose:''' Cloning of 1902 pT7A3-6H-sso7d-T7-DNA-Ligase '''Date: '''2013-10-22--- '''Notebook:0''' '''Page:''..." 2013-10-25T20:24:16Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 1902 pT7A3-6H-sso7d-T7-DNA-Ligase&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1902 pT7A3-6H-sso7d-T7-DNA-Ligase &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-10-22--- &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1902 pT7A3-6H-sso7d-T7-DNA-Ligase&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1902 pT7A3-6H-sso7d-T7-DNA-Ligase <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-10-22--- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1902&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> subclone T7 DNA ligase from 1464 pT7A3-T7 DNA ligase or 1037 p8H-T7ligase to 1846 pT7A3-6H-sso7d using Nde1 + Xho1 <br /> <br /> [[Image:DIGEST FOR 1900 1902.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-10-24 <br /> <br /> Make miniprep, digest with Nco1 + Nde1 + Xho1--&amp;gt; T4 DNAligase + sso7d bands&lt;br&gt; <br /> <br /> [[Image:1900_1901_1902_1903digest.JPG]]&lt;br&gt; <br /> <br /> stock1902-2&lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Paul.xu