https://eln.excellgen.com/index.php?action=history&feed=atom&title=1904_pT7A3-T3-DNA-Ligase-sso7d 1904 pT7A3-T3-DNA-Ligase-sso7d - Revision history 2026-10-18T03:21:54Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1904_pT7A3-T3-DNA-Ligase-sso7d&diff=10511&oldid=prev Paul.xu: Created page with "== '''Cloning of 1904 pT7A3-T3-DNA-Ligase-sso7de''' == '''Purpose:''' Cloning of 1904 pT7A3-T3-DNA-Ligase-sso7d '''Date: '''2013-10-22--- '''Notebook:0''' '''Page:''' '..." 2013-10-25T20:29:37Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 1904 pT7A3-T3-DNA-Ligase-sso7de&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1904 pT7A3-T3-DNA-Ligase-sso7d &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-10-22--- &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1904 pT7A3-T3-DNA-Ligase-sso7de&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1904 pT7A3-T3-DNA-Ligase-sso7d <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-10-22--- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1903&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> subclone T3 DNA ligase from 1096 pET28b-T3-Ligase or 1500 pT7A3-T3-ligase to 1847 pT7A30-sso7d-6H using Nde1 + Xho1 <br /> <br /> [[Image:DIGEST FOR 1903 1904.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-10-24 <br /> <br /> Make miniprep, digest with Nde1 + Xho1--&amp;gt; T3 DNAligase + sso7d bands&lt;br&gt; <br /> <br /> [[Image:1904DIGET.JPG]][[Image:1904DIGEST.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> stock 1904-1&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Paul.xu