https://eln.excellgen.com/index.php?action=history&feed=atom&title=1910-pT7A3E-BsuPol 1910-pT7A3E-BsuPol - Revision history 2026-10-18T05:50:57Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1910-pT7A3E-BsuPol&diff=12245&oldid=prev Excellgen: Created page with "== '''Cloning of 1910-pT7A3E-BsuPol''' == '''Purpose:''' Cloning of 1910-pT7A3E-BsuPol '''Date: '''2014-7-01---2014-7-02 '''Notebook:0''' '''Page:''' '''Clone ID:1910''..." 2014-07-07T20:29:31Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 1910-pT7A3E-BsuPol&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1910-pT7A3E-BsuPol &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-7-01---2014-7-02 &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clone ID:1910&#039;&#039;...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1910-pT7A3E-BsuPol&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1910-pT7A3E-BsuPol <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-7-01---2014-7-02 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1910&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; LIANGHUO <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> 1. PCR from genomic DNA&lt;br&gt;Fragment 1. &lt;br&gt;BsuPol-Nde1-F&lt;br&gt;ttaaacatatggatcttggcttcaacacgcttcttg<br /> <br /> BsuPol-R2 caacgtcgtcataagaattgccc<br /> <br /> [[Image:1910Fra1_PCR.JPG]]<br /> <br /> Fragment 2&lt;br&gt;BsuPol-F2 gggcaattcttatgacgacgttg<br /> <br /> BsuPol-Xho1-R&lt;br&gt;(catcttggtacgatgcgaaa ctcgag agaatc)&lt;br&gt;gattctctcgagttatttcgcatcgtaccaagatg<br /> <br /> [[Image:1910Fra2_1535_PCR.JPG]]<br /> <br /> 2. Join two fragments&lt;br&gt;BsuPol-Nde1-F + BsuPol-Xho1-R<br /> <br /> [[Image:1910_full_length_PCR.JPG]]<br /> <br /> 3. Purify and digest with Nde1 + Xho1&lt;br&gt;<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; &lt;br&gt; <br /> <br /> === 1910 DIGESTED ===<br /> <br /> [[Image:1535_1910_2212_2216_Digested.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014-7-02 <br /> <br /> Make miniprep and digest with Nde1 + XhoI <br /> <br /> [[Image:1274_1910_1535_2212_2230_Screening.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> SELECT CLONE2 FOR SEQUENCING&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div> Excellgen