https://eln.excellgen.com/index.php?action=history&feed=atom&title=1915-pT7A3-8H-HC-VV-PolyA-Pol_S 1915-pT7A3-8H-HC-VV-PolyA-Pol S - Revision history 2026-10-18T20:14:02Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1915-pT7A3-8H-HC-VV-PolyA-Pol_S&diff=11110&oldid=prev Paul.xu: Created page with "== '''Cloning of 1915-pT7A3-8H-HC-VV-PolyA-Pol S''' == '''Purpose:''' Cloning of 1915-pT7A3-8H-HC-VV-PolyA-Pol S '''Date: '''2014-1-23--2014-1-27 '''Notebook:0''' '''Page:'..." 2014-01-28T16:08:48Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 1915-pT7A3-8H-HC-VV-PolyA-Pol S&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1915-pT7A3-8H-HC-VV-PolyA-Pol S &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-1-23--2014-1-27 &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1915-pT7A3-8H-HC-VV-PolyA-Pol S&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1915-pT7A3-8H-HC-VV-PolyA-Pol S<br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-1-23--2014-1-27<br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1915&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1142_1672_1737_1915_2013pcr_1-23-2014.JPG]]<br /> <br /> PCR from vaccinia virus genomic DNA using VV-PolyA-Pol_S-Nde1-F + VV-PolyA-Pol_S-HindIII-R&lt;br&gt;<br /> <br /> [[Image:1672_1737_1915_1940clone_1-24-2014.JPG]]&lt;br&gt;<br /> <br /> {| width=&quot;200&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt;<br /> | 5&lt;br&gt;<br /> | 6&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | pcr &lt;br&gt;<br /> | 1725 pT7A3-8H&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | colspan=&quot;2&quot; | Nde1 +HindIII&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt;<br /> <br /> &lt;br&gt;<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014-1-27<br /> <br /> Make miniprep and digest with Nde1 +HindIII<br /> <br /> [[Image:1915_1940_2039digest_1-27-2014.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt;&lt;br&gt; [[Category:Cloning]]</div> Paul.xu