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1916 pT7A3E-HC2-M-SssI - Revision history
2026-10-18T16:59:10Z
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Paul.xu at 15:30, 23 June 2018
2018-06-23T15:30:52Z
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<p><b>New page</b></p><div>== '''Cloning of 1916 pT7A3E-HC2-M-SssI''' ==<br />
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'''Purpose:''' Cloning of1916 pT7A3E-HC2-M-SssI'''=='''<br />
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'''Date: '''2014-9-15---<br />
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'''Notebook:0'''<br />
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'''Page:'''<br />
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'''Clone ID:1916'''<br />
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'''Experimenters:'''&nbsp; shanyi<br />
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== '''Results''' ==<br />
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=== CLONING ===<br />
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gene synthesis<br />
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[[File:1916 2326 2327 2328 2199pcr.JPG|RTENOTITLE]]<br />
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join two fragments by PCR<br />
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Digest with Nde1 + Xho1<br />
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=== Colony Screening ===<br />
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Date: 2014_9<br />
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Make miniprep, use Nde1 + Xho1 to find positive clone<br />
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== '''Result intepretation/Summary''' ==<br />
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*<br />
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== '''Conclusion''' ==<br />
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'''===='''<br />
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'''1916 in pMiniT2'''<br />
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'''6/4/2018, gene synthesis'''<br />
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'''digest with EcoR1, submit&nbsp;#3 for sequencing'''<br />
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'''[[File:New 1916 Ecor1.JPG|RTENOTITLE]]'''<br />
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1916 amplified by combining two fragments: one from above, another from IDT gene fragment<br />
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miniprep digest with EcoR1:<br />
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[[File:New1916 pminiT EcoR1.JPG]]<br />
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6/22/18: #2 sequencing : missing&nbsp;missing GTT between tow fragments<br />
[[Category:Cloning]]</div>
Paul.xu