https://eln.excellgen.com/index.php?action=history&feed=atom&title=1924-pT7A3E-phi29e-HI 1924-pT7A3E-phi29e-HI - Revision history 2026-10-18T21:36:51Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1924-pT7A3E-phi29e-HI&diff=10839&oldid=prev Paul.xu: Created page with "== '''Cloning of 1924-pT7A3E-phi29e-HI''' == '''Purpose:''' Cloning of 1924-pT7A3E-phi29e-HI '''Date: '''2013-12-17---2013-12-19 '''Notebook:0''' '''Page:''' '''Clone ID:..." 2013-12-20T16:43:50Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 1924-pT7A3E-phi29e-HI&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1924-pT7A3E-phi29e-HI &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-12-17---2013-12-19 &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clone ID:...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1924-pT7A3E-phi29e-HI&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1924-pT7A3E-phi29e-HI<br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-12-17---2013-12-19<br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1924&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1924+1925clone_12-17-2013.JPG]]<br /> <br /> {| width=&quot;391&quot; height=&quot;83&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt;<br /> | 1&lt;br&gt;<br /> | 2&lt;br&gt;<br /> | 3&lt;br&gt;<br /> | 4&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | pcr Q5&lt;br&gt;<br /> | 1923 pT7A3E-HI&lt;br&gt;<br /> | PCR phusion&lt;br&gt;<br /> | 1923 pT7A3E-HI&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | Nde1-Sal1&lt;br&gt;<br /> | Nde1-Xho1&lt;br&gt;<br /> | Nde1-Sal1&lt;br&gt;<br /> | Nde1-Xho1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt;<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-12-19<br /> <br /> Make miniprep and digest with Nde1+Eag1&lt;br&gt;<br /> <br /> [[Image:1924digest_12-19-2013.JPG]]<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; [[Category:Cloning]]</div> Paul.xu