https://eln.excellgen.com/index.php?action=history&feed=atom&title=1925-pT7A3-phi29wt-HI 2026-10-18T19:43:29Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1925-pT7A3-phi29wt-HI&diff=10836&oldid=prev 2013-12-20T14:13:03Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1925-pT7A3-phi29wt-HI&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1925-pT7A3-phi29wt-HI <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-12-17---2013-12-19 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1925&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1924+1925clone_12-17-2013.JPG]]&lt;br&gt;<br /> <br /> {| width=&quot;292&quot; height=&quot;83&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt;<br /> | 5&lt;br&gt;<br /> | 6&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | 549 pET28b-Phi29DNAPol &lt;br&gt;<br /> | 1923 pT7A3E-HI&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | colspan=&quot;2&quot; | Xba1 + Xho1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt;<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-12-19 <br /> <br /> Make miniprep and digest with Xba1 + Xho1<br /> <br /> [[Image:1925_1926_1931digest_12-19-2013.JPG]]&lt;br&gt; <br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div>

Paul.xu