https://eln.excellgen.com/index.php?action=history&feed=atom&title=1927_pT7A3-T4-DNA-Ligase-sso7d-6H 1927 pT7A3-T4-DNA-Ligase-sso7d-6H - Revision history 2026-10-18T13:45:05Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1927_pT7A3-T4-DNA-Ligase-sso7d-6H&diff=10687&oldid=prev Paul.xu: Created page with "== '''Cloning of 1927 pT7A3-T4-DNA-Ligase-sso7d-6H''' == '''Purpose:''' Cloning of 1927 pT7A3-T4-DNA-Ligase-sso7d-6H '''Date: '''2013-11-24--- '''Notebook:0''' '''Page:''..." 2013-11-27T19:30:24Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 1927 pT7A3-T4-DNA-Ligase-sso7d-6H&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1927 pT7A3-T4-DNA-Ligase-sso7d-6H &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-11-24--- &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1927 pT7A3-T4-DNA-Ligase-sso7d-6H&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1927 pT7A3-T4-DNA-Ligase-sso7d-6H <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-11-24--- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1927&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === PCR ===<br /> <br /> PCR from 240 or 1483 <br /> <br /> [[Image:1927_1928_1930pcr.JPG‎ ]]&lt;br&gt; digest with NdeI+XhoI <br /> <br /> [[Image:2digest_for1927_1928_1930.JPG‎ ]]&lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-11-27 <br /> <br /> Make miniprep, digest with NdeI+XhoI&lt;br&gt; <br /> <br /> [[Image:1927 1928digest.JPG]]<br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Paul.xu