https://eln.excellgen.com/index.php?action=history&feed=atom&title=1929-pT7A3E-Ecoli-Ligase 1929-pT7A3E-Ecoli-Ligase - Revision history 2026-10-18T22:24:33Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1929-pT7A3E-Ecoli-Ligase&diff=10628&oldid=prev Paul.xu: Created page with "== '''Cloning of 1929-pT7A3E-Ecoli-Ligase''' == '''Purpose:''' Cloning of 1929-pT7A3E-Ecoli-Ligase '''Date: '''2013-11-12---2013-11-14 '''Notebook:0''' '''Page:''' '''Clo..." 2013-11-15T14:58:56Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 1929-pT7A3E-Ecoli-Ligase&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1929-pT7A3E-Ecoli-Ligase &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-11-12---2013-11-14 &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clo...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1929-pT7A3E-Ecoli-Ligase&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1929-pT7A3E-Ecoli-Ligase<br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-11-12---2013-11-14<br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1929&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1878+1929clone_11-12-2013.JPG]]&lt;br&gt; <br /> <br /> {| width=&quot;289&quot; height=&quot;80&quot; border=&quot;1&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt;<br /> | 3&lt;br&gt;<br /> | 4&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | 1134,pT7-Ecoli-Ligase&lt;br&gt;<br /> | 1892 pT7A3E&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | colspan=&quot;2&quot; | Nde1 + Xho1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt;<br /> <br /> <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-11-14<br /> <br /> Make miniprep and digest with&amp;nbsp; Nde1 + Xho1 <br /> <br /> [[Image:1929digest_11-14-2013.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Paul.xu