https://eln.excellgen.com/index.php?action=history&feed=atom&title=1935-pT7A3E-Bst1-Exo-HI 1935-pT7A3E-Bst1-Exo-HI - Revision history 2026-10-18T13:43:43Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1935-pT7A3E-Bst1-Exo-HI&diff=10945&oldid=prev Paul.xu: Created page with "== '''Cloning of 1935-pT7A3E-Bst1-Exo-HI''' == '''Purpose:''' Cloning of 1935-pT7A3E-Bst1-Exo-HI '''Date: '''2014-1-2---2014-1-9 '''Notebook:0''' '''Page:''' '''Clone ID:..." 2014-01-14T21:17:12Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 1935-pT7A3E-Bst1-Exo-HI&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1935-pT7A3E-Bst1-Exo-HI &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-1-2---2014-1-9 &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clone ID:...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1935-pT7A3E-Bst1-Exo-HI&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1935-pT7A3E-Bst1-Exo-HI<br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-1-2---2014-1-9<br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1935&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1315_1935_1943_1946_1984PCR_12-31-2013.JPG]]<br /> <br /> Bst-Exo-Nde1-F &lt;br&gt;Bst-Exo-Xho1-R &lt;br&gt;template is 1144&lt;br&gt;<br /> <br /> [[Image:1935_1984clone_1-7-2014.JPG]]&lt;br&gt;<br /> <br /> {| width=&quot;200&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt;<br /> | 1&lt;br&gt;<br /> | 2&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | pcr&lt;br&gt;<br /> | 1923 pT7A3E-HI&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | colspan=&quot;2&quot; | Nde1 + Xho1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt;<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014-1-9<br /> <br /> Make miniprep and digest with Nde1 + Xho1 <br /> <br /> [[Image:1953_1974digest_1-9-2014.JPG]]&lt;br&gt; <br /> <br /> 1935 not 1953&lt;br&gt;<br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; [[Category:Cloning]]</div> Paul.xu