https://eln.excellgen.com/index.php?action=history&feed=atom&title=1938_pT7A3E-Klenow35exo 2026-10-18T21:36:51Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1938_pT7A3E-Klenow35exo&diff=11052&oldid=prev 2014-01-24T15:54:40Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1938 pT7A3E-Klenow35exo&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1938 pT7A3E-Klenow35exo&#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-1---- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1938&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === subcloning ===<br /> <br /> subclone Klenow(3&#039;-&amp;gt;5&#039;_exo–) from 1530 to 1892(-1) pT7A3E ,using Nde1 + HindIII <br /> <br /> [[Image:IDIGEST FOR 1937 1938 1939.JPG]]&lt;br&gt; <br /> <br /> [[Image:Digest vectors for 1886 1887 1938 1939 1947 1993 1994 1998 2000 1535 1588.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-10-23 <br /> <br /> Make miniprep, digest with NdeI+XhoI &lt;br&gt; <br /> <br /> [[Image:1938_1939_1947_1993digest1.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div>

Paul.xu