https://eln.excellgen.com/index.php?action=history&feed=atom&title=1940-pLysN-ha-Plac-Hia5 2026-10-18T08:20:54Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1940-pLysN-ha-Plac-Hia5&diff=11304&oldid=prev 2014-02-21T19:03:43Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1940-pLysN-ha-Plac-Hia5&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1940-pLysN-ha-Plac-Hia5 <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-1-8---2014-1-13 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1940&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:724 725 1940pcr 1-9-2014.JPG]] <br /> <br /> 1838 pEC-hia5&lt;br&gt;ha-constitutive-PLac-F &lt;br&gt;ha-constitutive-PLac-Hia5-F&lt;br&gt; <br /> <br /> [[Image:724 725 1940 1946 1961clone 1-9-2014.JPG]]&lt;br&gt; <br /> <br /> {| width=&quot;328&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt; <br /> | 5&lt;br&gt; <br /> | 6&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | pcr&lt;br&gt; <br /> | 674 pLysN-Plac-mXho1&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | colspan=&quot;2&quot; | &amp;nbsp;&amp;nbsp;&amp;nbsp; HindIII + Xho1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014-1-13 <br /> <br /> Make miniprep and digest with HindIII + Xho1 <br /> <br /> [[Image:1940digest 1-13-2014.jpg]]&lt;br&gt; <br /> <br /> [[Image:1940pcr screen 1-14-2014.jpg]] <br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;subCloning of 1940-pLysN-ha-Plac-Hia5&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1940-pLysN-ha-Plac-Hia5 <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-2-12---2014-2-14 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1940&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1114 1142 1940 2016clone 2-12-2014.JPG]]&lt;br&gt; <br /> <br /> {| width=&quot;328&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt; <br /> | 5&lt;br&gt; <br /> | 6&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | 2069,pEC-ha-Plac-hia5&lt;br&gt; <br /> | 674 pLysN-Plac-mXho1&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | colspan=&quot;2&quot; | &amp;nbsp;&amp;nbsp;&amp;nbsp; HindIII + Xho1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014-2-14 <br /> <br /> Make miniprep and digest with HindIII + Xho1 <br /> <br /> [[Image:1940 2016digest 2-14-2014.JPG]]&lt;br&gt; <br /> <br /> c:674 pLysN-Plac-mXho1&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> digest with HindIII +BamH1 &lt;br&gt; <br /> <br /> [[Image:1940digest2-29-2014.JPG]] <br /> <br /> &lt;br&gt;<br /> <br /> 2014-2-21&lt;br&gt;<br /> <br /> PCR Screen:pLysN-HindIII-seq-F2 &lt;br&gt;Hia5-Xho1-F&lt;br&gt;<br /> <br /> [[Image:1940pcr_screen_2-21-2014.JPG]]&lt;br&gt;<br /> <br /> c:674 pLysN-Plac-mXho1&lt;br&gt;<br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div>

Paul.xu