https://eln.excellgen.com/index.php?action=history&feed=atom&title=1942_pT7A3E-new-phi29 1942 pT7A3E-new-phi29 - Revision history 2026-10-18T16:58:50Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1942_pT7A3E-new-phi29&diff=10935&oldid=prev Paul.xu: Created page with "== '''Cloning of 1942 pT7A3E-new-phi29''' == '''Purpose:''' Cloning of1942 pT7A3E-new-phi29 '''Date: '''2014-1---- '''Notebook:0''' '''Page:''' '''Clone ID:1942''' ''..." 2014-01-13T18:24:40Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 1942 pT7A3E-new-phi29&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of1942 pT7A3E-new-phi29 &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-1---- &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clone ID:1942&#039;&#039;&#039; &#039;&#039;...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1942 pT7A3E-new-phi29&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of1942 pT7A3E-new-phi29 <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-1---- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1942&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> PCR using 1617 pT7A3-MBP-(3c)-newPhi29 as template<br /> <br /> New-Phi29-1-1 A1 ATCGACGCTAGCATGAAACATATGCCACGCAAAATG plate 1_10_2013<br /> <br /> newPhi29-Sal1-R (GACGATACCTTTACCATTAAGgtcgac tctaca) tgtagagtcgacCTTAATGGTAAAGGTATCGTC<br /> <br /> [[Image:1535_1588_1942_1995pcr.JPG]]<br /> <br /> digest vector&amp;nbsp;&#039;&#039;&#039;1892 pT7A3E&#039;&#039;&#039; with Nde1 + Xho1, Digest PCR product with Nde1 + Sal1&lt;br&gt; <br /> <br /> [[Image:Digest pcrfor 1561 1631 1693 1942 1995.JPG]]&lt;br&gt; <br /> <br /> [[Image:2digest vectors for 1561 1631 1693 1942 1995.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-10-23 <br /> <br /> Make miniprep, digest with NdeI+XHOI&lt;br&gt; <br /> <br /> [[Image:1561 1631 1693 1942 1995digest.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Paul.xu