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1944 pT7A3E-polyAPol - Revision history
2026-10-18T16:08:24Z
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Excellgen at 14:48, 29 April 2015
2015-04-29T14:48:38Z
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<p><b>New page</b></p><div>== '''Cloning of 1944 pT7A3E-polyAPol''' ==<br />
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'''Purpose:''' Cloning of 1944 pT7A3E-polyAPol'''==''' <br />
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'''Date: '''2014-1---- <br />
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'''Notebook:0''' <br />
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'''Page:''' <br />
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'''Clone ID:1944''' <br />
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'''Experimenters:'''&nbsp; shanyi <br />
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== '''Results''' ==<br />
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=== subcloning ===<br />
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subclone E coli poly(A) polymerase I from 1140 to 1892 pT7A3E ,using NdeI+XhoI<br />
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[[File:Digest for 1944 1950.JPG|RTENOTITLE]]<br />
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=== Colony Screening ===<br />
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Date: 2013-10-23<br />
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Make miniprep, digest with NdeI+XhoI<br />
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[[File:1944 1950 1992DIGEST.JPG|RTENOTITLE]][[File:1944 1950DIGEST.JPG|RTENOTITLE]]<br />
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== Subcloning and Screening ==<br />
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Digested by Nde1 and Xho1<br />
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[[File:1944 1513 digestion.JPG|RTENOTITLE]]<br />
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=== 1944 PCR product 4/23/2015 ===<br />
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[[File:1944 insert.JPG|RTENOTITLE]]<br />
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1944 Digestion by Nde1+Xho1&nbsp; 4/28/2015<br />
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[[File:1944 Digest.JPG]]<br />
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== '''Result intepretation/Summary''' ==<br />
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*<br><br />
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== '''Conclusion''' ==<br />
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[[Category:Cloning]]</div>
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