https://eln.excellgen.com/index.php?action=history&feed=atom&title=1946-pT7A3E-bTdT 1946-pT7A3E-bTdT - Revision history 2026-10-18T06:31:56Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1946-pT7A3E-bTdT&diff=10969&oldid=prev Paul.xu: Created page with "== '''Cloning of 1946-pT7A3E-bTdT''' == '''Purpose:''' Cloning of 1946-pT7A3E-bTdT '''Date: '''2014-1-5---2014-1-13 '''Notebook:0''' '''Page:''' '''Clone ID:1946''' ''..." 2014-01-15T14:24:40Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 1946-pT7A3E-bTdT&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1946-pT7A3E-bTdT &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-1-5---2014-1-13 &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clone ID:1946&#039;&#039;&#039; &#039;&#039;...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1946-pT7A3E-bTdT&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1946-pT7A3E-bTdT <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-1-5---2014-1-13<br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1946&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1146_1315_1946pcr_12-30-2013.JPG]]<br /> <br /> pcr1:bTdT-1-1 to bTdT-1-14&lt;br&gt;<br /> <br /> pcr2:bTdT-2-1 to bTdT-2-14&lt;br&gt;<br /> <br /> [[Image:1315_1935_1943_1946_1984PCR_12-31-2013.JPG]]&lt;br&gt;<br /> <br /> PCR :pcr1+pcr2&lt;br&gt;<br /> <br /> [[Image:724_725_1940_1946_1961clone_1-9-2014.JPG]]&lt;br&gt;<br /> <br /> {| width=&quot;227&quot; height=&quot;67&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt;<br /> | &lt;br&gt;<br /> | &lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | pcr&lt;br&gt;<br /> | 1892 pT7A3E&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | colspan=&quot;2&quot; | Nde1 + Xho1 &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt;<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014-1-14<br /> <br /> Make miniprep and digest with Nde1 + Xho1 <br /> <br /> [[Image:1946_1961digest_1-13-2014.jpg]]&lt;br&gt; <br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; [[Category:Cloning]]</div> Paul.xu