https://eln.excellgen.com/index.php?action=history&feed=atom&title=1949_pT7A3-Kfu-sso7d 1949 pT7A3-Kfu-sso7d - Revision history 2026-10-18T05:02:35Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1949_pT7A3-Kfu-sso7d&diff=16770&oldid=prev Paul.xu: Paul.xu moved page 1949 pT3A3-Kfu-sso7d to 1949 pT7A3-Kfu-sso7d 2018-01-13T20:22:28Z <p>Paul.xu moved page <a href="/index.php?title=1949_pT3A3-Kfu-sso7d" class="mw-redirect" title="1949 pT3A3-Kfu-sso7d">1949 pT3A3-Kfu-sso7d</a> to <a href="/index.php?title=1949_pT7A3-Kfu-sso7d" title="1949 pT7A3-Kfu-sso7d">1949 pT7A3-Kfu-sso7d</a></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1949 pT3A3-Kfu-sso7d&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1949 pT3A3-Kfu-sso7d <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-12-4--- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1949&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> PCR &lt;br&gt; <br /> <br /> PCR two fragments from Kod 1801 pT7A30-KOD-sso7d&lt;br&gt;1&lt;br&gt;Kfu-F1+Kfu-R2&lt;br&gt;2 <br /> <br /> Kfu-F3 c+Kfu-R4&lt;br&gt;&lt;br&gt;PCR 1 fragment from Pfu 1715 pT7A30-Pfu-sso7d <br /> <br /> 3&lt;br&gt;Kfu-F2+Kfu-R3&lt;br&gt;[[Image:1949PCR1.JPG]][[Image:1949PCR2 3.JPG]]&lt;br&gt; <br /> <br /> Join three fragments using <br /> <br /> Kfu-F1+Kfu-R4 <br /> <br /> [[Image:1949PCR.JPG]]&lt;br&gt;digest with Nde1 + Xho1 and clone into Nde1 + Xho1 digested vector 1847 pT7A30-sso7d-6H <br /> <br /> [[Image:DIGEST FOR 1949.JPG]]&lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-12-6 <br /> <br /> Make miniprep, digest with NdeI+XhoI&lt;br&gt; <br /> <br /> [[Image:1941 1949digest.JPG]] <br /> <br /> send 1949-2 to sequence with T7term primer&lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Paul.xu