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2026-10-18T19:26:12Z
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Paul.xu at 21:12, 8 January 2014
2014-01-08T21:12:40Z
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<p><b>New page</b></p><div>== '''Cloning of 1961-pCMV5-HTNCre''' ==<br />
<br />
'''Purpose:''' Cloning of 1961-pCMV5-HTNCre <br />
<br />
'''Date: '''2013-12-30---2-14-1-7 <br />
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'''Notebook:0''' <br />
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'''Page:''' <br />
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'''Clone ID:1961''' <br />
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'''Experimenters:''' yingying <br />
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== '''Results''' ==<br />
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=== cloning ===<br />
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[[Image:1961+1962pcr_12-20-2013.JPG]]<br><br />
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1. PCR His-Tat-NLS from 1708 pT7A3-HTNCre<br />
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pcr1<br />
<br />
1708 pT7A3-HTNCre<br />
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6His-Nhe1-F<br>NLS-HindIII-R<br />
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PCR2 Flpo from 1520 pDIRE<br>NLS-HindIII-F<br>iCre-Eag1-Kpn1-R<br />
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PCR Then join two fragments<br />
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6His-Nhe1-F<br />
<br />
iCre-Eag1-Kpn1-R<br><br />
<br />
[[Image:715_718_1961PCR_12-27-2013.JPG]]<br><br />
<br />
[[Image:1948_1961_1962clone_12-30-2013.JPG]]<br><br />
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{| width="334" height="116" cellspacing="1" cellpadding="1" border="1"<br />
|-<br />
| lane<br><br />
| 3<br><br />
| 4<br><br />
|-<br />
| <br><br />
| pcr<br><br />
| 1758 pCMV5-hIgG-Lc-FN3K<br><br />
|-<br />
| <br><br />
| Nhe1 + Kpn1<br><br />
| <br><br />
|}<br />
<br />
<br><br />
<br />
=== Colony Screening ===<br />
<br />
Date: 2014-1-6 <br />
<br />
Make miniprep and digest with Nhe1 + Kpn1/hind111 + Kpn1<br />
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[[Image:1961_1962digest_1-6-2014.JPG]][[Image:1961_1962digest_1-7-2014.JPG]]<br><br />
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<br> <br />
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<br> <br />
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== '''Result intepretation/Summary''' ==<br />
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== '''Conclusion''' ==<br />
<br />
<br> <br />
<br />
[[Category:Cloning]]</div>
Paul.xu