https://eln.excellgen.com/index.php?action=history&feed=atom&title=1966-pCMV5-c-HA 1966-pCMV5-c-HA - Revision history 2026-10-18T10:30:32Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1966-pCMV5-c-HA&diff=11093&oldid=prev Paul.xu: Created page with "== '''Cloning of 1966-pCMV5-c-HA''' == '''Purpose:''' Cloning of 1966-pCMV5-c-HA '''Date: '''2014-1-15---2014-1-24 '''Notebook:0''' '''Page:''' '''Clone ID:1966''' '''E..." 2014-01-28T15:28:51Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 1966-pCMV5-c-HA&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1966-pCMV5-c-HA &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-1-15---2014-1-24 &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clone ID:1966&#039;&#039;&#039; &#039;&#039;&#039;E...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1966-pCMV5-c-HA&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1966-pCMV5-c-HA<br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-1-15---2014-1-24<br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1966&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1954_1964_1965_1966_1981_2019pcr_1-16-2014.jpg]]<br /> <br /> genge synthesis&lt;br&gt;<br /> <br /> c_HA-1 to c_HA-6&lt;br&gt;<br /> <br /> [[Image:1954_1964_1966_1945_1979_2019clone_1-16-2014.jpg]]&lt;br&gt;<br /> <br /> {| width=&quot;286&quot; height=&quot;100&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt;<br /> | 5&lt;br&gt;<br /> | 6&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | pcr&lt;br&gt;<br /> | 1969 pCMV5-Gag-p2b-NLS-Flp-WPRE-oriP&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | colspan=&quot;2&quot; | Kpn1 + EcoR1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt;<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014-1-24 <br /> <br /> Make miniprep and digest with Kpn1 + EcoR1&lt;br&gt;<br /> <br /> [[Image:1954_1964_1966digest_1-24-2014.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt;&lt;br&gt; [[Category:Cloning]]</div> Paul.xu